| Congresso Brasileiro de Microbiologia 2023 | Resumo: 827-1 | ||||
Resumo:Microbial oils, also known as single cell oils (SCOs), are considered potential substitutes for vegetable oils due to their similarities in fatty acid composition, combined with the reduction of environmental damage resulting from the pursuit of sustainable processes. Some yeast species can accumulate up to 70% w/w of lipids relative to their dry biomass and have advantages for industrial-scale implementation. To reduce costs related to microbial cultivation, alternative carbon sources such as raw glycerol are used. The aim of this study was to select new yeast strains from the Collection of Agricultural Microbiology (CCMA) at the Federal University of Lavras for lipid production using raw glycerol as a substrate. Twenty-six yeast strains were evaluated through submerged cultivation in medium containing 40g/L of raw glycerol. Cultures with an inoculum of 107 cells/mL were incubated in an orbital shaker at 28°C with agitation at 150 rpm for 96 hours. At the end of the cultivation, 100 mL samples were taken, the biomass was dried, weighed, and then subjected to lipid extraction using chloroform and methanol solvents. The lipid content (%) was determined by calculating the extracted content relative to the weight of the dry biomass. The strains Rhodotorula dairenensis CCMA 0945 (21.21%), Exophiala spinifera CCMA 2073 (23.97%), Cryptococcus laurentii Palavras-chave: fermentation processes, oleaginous yeast, lipids, raw glycerol Agência de fomento:CNPq, CAPES | |||||